Figure 5.

Distinct involvement of the transcription factor p53 after single-agent and combined stimulation. (a) JURKAT cells stimulated with doxorubicin and BA as indicated were fractionated into cytosolic and nuclear extracts for western blot. GAPDH and Histon H1 served as loading control. (b) Parental JURKAT cells and JURKAT cells stably transfected with a control sequence (mock) or shRNA against p53 (shp53) were stimulated with doxorubicin and BA. (c) JURKAT cells from Figure 5b were evaluated for the expression level of NOXA as in Figure 4a. (d) The experimental setting from Figure 5b of shRNA interference against p53 was applied for the drug combinations of doxo and BA as in Figure 4c. Data for parental JURKAT cells are presented in Figure 4c. Drug concentrations, experimental procedure, measurement of apoptosis induction, statistical analysis and presentation of data were identical as in Figure 1a and Supplementary Figure S1 if not stated differently