Figure 5.
hIL-10 does not influence ODN CpG-2006-induced cell death. Akata cells and DN-MyD88 Akata cells were incubated with medium only, 0.5 μM ODN CpG-2006, 900 pg/ml rhIL-10 or combination of ODN CpG-2006 and rhIL-10. To one ODN CpG-2006-stimulated sample, 0.5 mM ODN CpG-2006 was added again after 48 h (rep. CpG-2006). (a) The cell viability was monitored by the Trypan Blue exclusion test 0, 48 and 72 h after treatment. (b) After 72 h cells were harvested and 105 cells were stained with PE-Annexin V/7-AAD and analyzed by flow cytometry. (c) Quantification of (b)