Figure 4.

Knockdown of Twist1 knockdown inhibits mTOR-mediated Mcl-1 downregulation. (a) H1299 cells were transfected with two different Twist1 or control siRNAs for 30 h. (b) H1299 cells were transfected with control or mTOR siRNA for 30 h. (c) H1299 cells were transfected with control or mTOR siRNA for 20 h, followed by treatment with 5 μM cisplatin for 24 h. Cell viability was measured with the MTT assay (c). The viability of control siRNA (siCTL)-treated group was set at 100%, and survival relative to the siCTL presented. The indicated protein levels were measured using western blot analysis (a–c). The blot shown is representative of two independent experiments. Cell viability data are shown as the mean of triplicate samples, and error bars reflect S.D. *P<0.05 versus the siCTL/cisplatin-treated group or simTOR-treated group