Figure 5.

Notch2 signaling controls proliferation in GSC-like cells. (a) Compared to spheres derived from the parental U373 cell line (WT), spheres overexpressing N2ICD are increased in size (N2ICD), indicating increased proliferation of GSC-like cells. In contrast, spheres derived from U373 cells expressing MAML1dn, which inhibits canonical Notch signaling, were smaller in size (MAML1dn). (b) Quantification shows that U373-N2ICD spheres have twice the diameter compared with U373-WT spheres, whereas U373-MAML1dn spheres show a decrease in diameter by 25%. (c) Immunostaining of U373-N2ICD spheres for GSC markers (Nestin, Sox2 and CD133) confirms that the spheres retain stem-like properties. Box plot diagrams show median (red line), boxed 25 and 75% percentiles, while whiskers mark minima and maxima (Mann–Whitney test, P<0.0001). Scale bar (a)=100 μm, (c)=50 μm