Figure 3.

Oxidative stress induced by H₂O₂ induces OPC detachment and death, an effect that was attenuated by CBD by decreasing ROS production. (a) Phase contrast images ( × 10) show that treatment with H₂O₂ induced the swelling and detachment of OPCs, an effect prevented by CBD treatment. (b) CBD protects OPCs from oxidative stress. OPCs were treated with H₂O₂ in the presence or absence of CBD (1 μM) and cell death was quantified 18 h later using the LDH method. The data represent the mean±S.E.M. of four independent cultures analyzed in triplicate, and the statistical significance was determined using one-way ANOVA followed by the Bonferroni post-hoc test: ***P⩽0.001 versus untreated cells, ^##P⩽0.01 versus cells exposed to H₂O₂ alone. (c) CBD decreased ROS production under conditions of oxidative stress. OPCs were loaded with DCF-DA for 30 min and stimulated with H₂O₂ in the presence or absence of CBD (1 μM). Fluorescence was measured 2 h later at 485/530 nm in a microplate reader and the data represent the means±S.E.M. of n=3 independent cultures analyzed in triplicate. Statistical significance was determined using Kruskal–Wallis ANOVA followed by Mann–Whitney U test: ***P⩽0.001 versus untreated cells, ^###P⩽0.001 versus cells exposed to H₂O₂ alone