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. 2011 Nov 8;4(2):201–212. doi: 10.1159/000332142

Fig. 5.

Fig. 5

PSM-induced CD11b upregulation is inhibited by the FPR2/ALX antagonist FLIPr. CD11b upregulation of neutrophils induced by formylated PSM peptides of S. aureus (PSMα2 1 μM, PSMα3 500 nM, δ-toxin 250 nM) or S. epidermidis (PSMα 500 nM, PSMδ 500 nM, and PSMε 500 nM) with and without FLIPr preincubation (a) and culture filtrates from S. epidermidis 1457 (0.75%), S. aureus RN6390 (0.75%), and USA300 (0.09%) or the isogenic Agr mutants (b). CD11b upregulation of neutrophils with and without FLIPr preincubation induced by culture filtrates from S. aureus RN6390 and the isogenic Agr mutant (0.75%) (c) or USA300 and USA300 ∆agr (0.09%) (d). Data represent means ± SEM of 3 independent experiments with neutrophils of different individuals and at least 3 different culture filtrates. * p < 0.05; *** p < 0.001. HSA = Human serum albumin; n.s. = not significant; S.a. = S. aureus; S.e. = S. epidermidis; TSB = tryptic soy broth.