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Effect of 200 ng/mL HMGB1 on ERK1/2 phosphorylation in NRVMs. NRVMs were pre-incubated with CsA for 1 h, and then incubated with 200 ng/mL HMGB1 for 2 h. ERK1/2 phosphorylation in NRVMs was determined by Western blot analysis. The graph shows fold changes in phosphorylated ERK1/2 (■) and total ERK1/2 (□). The bar graph represents three independent experiments. *P < 0.05 versus 200 ng/mL HMGB1 treated group. Ctrl: control group; CsA: cyclosporin A.
