Figure 4.

Characterization of the inhibitory effect of blocks 3 and 4. (a) Representation of plasmids containing blocks 3 and 4 inserted in both orientations upstream to the CMV-TK promoter, controlling the firefly luciferase reporter gene. (b) Luciferase activity was measured following transfection into the beta cell lines MIN6 and HIT, the alpha cell line αTC1, and the non-pancreatic cell line NIH-3T3. Activity is expressed relative to luciferase activity of the promoter-less vector pGL3-basic. Each data point represents mean ± SEM (n ≥ 3; *P < 0.05, as compared to the activity of CMV-TK promoter).