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. 2012 Jun;9(2):85–89. doi: 10.1089/zeb.2011.0731

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 2. — Comparative whole-mount in situ hybridization (WISH) staining of 32 hpf wild-type EK strain (A, B, I, J, Q, R), Tg(fli1a:EGFP)^y1 (C, D, K, L, S, T), Tg(flk:EGFP)^la116;Tg(gata1:DsRed)^sd2 (E, F, M, N, U, V) and Tg(Huc:EGFP) (G, H, O, P, W, X) embryos using tnnt2 probes generated from pCRII-TOPO vector (A–H), pGEM-T vector (I–P), or PCR-based template (Q–X). Panels B, D, F, H, J, L, N, P, R, T, V, and X are magnified views of the trunk regions of the embryos in panels A, C, E, G, I, K, M, O, Q, S, U, and W, respectively. Red arrowheads indicate off-target staining in axial vessels such as dorsal aorta and posterior cardinal vein. Green arrows show off-target staining in intersegmental vessels. Blue arrowhead indicates nonspecific staining by pGEM-T vector in the head of Tg(fli1a:EGFP)^y1 embryo (see Fig. 3K). Magenta arrows note off-target staining in the posterior blood island.