TABLE 2.
Strategies for the choice of experimental conditions in the global proton linkage analysis.
| pH 6.0 | pH 6.5 | pH 7.0 | pH 7.5 | pH 8.0 | pH 8.5 | |
|---|---|---|---|---|---|---|
| Strategy1 (1 pH, 4 buffers) | PBS, 298K | |||||
| HEPES, 298K | ||||||
| Imidazole,298K | ||||||
| Tris, 298K | ||||||
|
| ||||||
| Strategy2 (2 pHs, 6 buffers) | Cacodylate,298K | PBS, 298K | ||||
| MES, 298K | HEPES, 298K | |||||
| Bis-tris, 298K | Tris, 298K | |||||
|
| ||||||
| Strategy3 (3 pHs, 6 buffers) | Cacodylate,298K | PBS, 298K | CHES, 298K | |||
| MES, 298K | HEPES, 298K | HEPES,298K | ||||
| Bis-tris, 298K | Tris, 298K | Tris, 298K | ||||
|
| ||||||
| Strategy4 (6 pHs, 6 buffers, 3 Ts) | Cacodylate,298K | Bis-tris, 283K | Imidazole,298K | PBS, 283K | Tris,298K | HEPES,283K |
| Bis-tris, 298K | PBS, 298K | HEPES,298K | ||||
| Bis-tris, 310K | PBS, 310K | HEPES,310K | ||||
|
| ||||||
| Strategy5 (6 pHs, 8 buffers) | Cacodylate,298K | Cacodylate,298K | Imidazole,298K | PBS, 298K | Tris, 298K | CHES, 298K |
| MES, 298K | HEPES, 298K | HEPES,298K | ||||
| Bis-tris, 298K | Tris, 298K | Tris, 298K | ||||