Figure 5.

The inhibition of iNOS, COX-2, and HO-1 protein expression by AHT induced by Carr of foot at 5th hr in mice. Tissue suspended was then prepared and subjected to Western blotting using an antibody specific for iNOS and COX-2. β-actin was used as an internal control. (a) A representative Western blot from two separate experiments was shown. (b) Relative iNOS, COX-2, and HO-1 protein levels were calculated with reference to a Carr-injected mouse. ^###Compared with sample of control group. The data were presented as mean ± S.D. for three different experiments performed in triplicate. ***P < 0.001 was compared with the Carr-alone group (one-way ANOVA followed by Scheffe's multiple range test).