Table 1.

Primers used for real-time PCR analysis.

Gene	Primers	Annealing temperature (°C)
ALP	Forward: 5′-AGGCAGGATTGACCACGG-3′	58
	Reverse: 5′-TGTAGTTCTGCTCATGGA-3′
OPN	Forward: 5′-GACGGCCGAGGTGATAGCTT-3′	63
	Reverse: 5′-CATGGCTGGTCTTCCCGTTGC-3′
OC	Forward: 5′-AAAGCCCAGCGACTCTC-3′	59
	Reverse: 5′-CTAAACGGTGGTGCCATAGAT-3′
GAPDH	Forward: 5′-AACCCATCACCATCTTCCAGG-3′	60
	Reverse: 5′-GCCTTCTCCATGGTGGTGAA-3′