Figure 5.

Combination therapy inhibited tumour growth of lung cancer in vivo. (A) Treatment schedule in vivo. H460 and A549 at 1.0 × 10⁶ cells were inoculated subcutaneously into right posterior legs of nude mice. Treatment was initiated when tumours were palpable. Mice were treated with 200 μg kg⁻¹ GSI XX injected intraperitoneally 3 days per week after 8 Gy of radiation once a week. Tumour size was measured every 2 days. Some tumours were removed on day 15 and the expression of NICD3 was examined by western blot analysis. (B) Combination treatment showed the significant delay of tumour growth, compared with control or single treatment alone (n=5). (C) NICD3 expression was upregulated in H460 and A549 tumour treated with radiation. In contrast, combination therapy reduced NICD3 expression.