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. 2012 Jun 15;2(6):607–617. doi: 10.7150/thno.4611

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Characterization of FBEM-scVEGF conjugates and [¹⁸F]FBEM-scVEGF. (A) Inhibition of Cy5.5-scVEGF (Red) binding to VEGFR2 overexpressed on PAE/KDR cells by different concentrations of non-labeled scVEGF and FBEM-conjugated scVEGF. (B) The IC₅₀ values were 19.98±5.39 µg/ml and 13.99±1.80 µg/ml for FBEM-scVEGF and scVEGF, respectively as determined by relative fluorescence units derived from images in (A) by Image J software. (C) In gel autoradiography showed that ¹⁸F-FBEM-scVEGF existed as a mixture of monomer and dimer. (a-d) showed scVEGF protein and [¹⁸F]FBEM-scVEGF stained with Coomassie blue (a and b, respectively), or autoradiography (c and d, respectively). (D) Cell uptake, internalization and efflux (E) assay of [¹⁸F]FBEM-scVEGF on PAE/KDR cells. Data were from 2 experiments with triplicate samples and are expressed as mean ± SD. AD, total added dose.