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. Author manuscript; available in PMC: 2012 Jul 3.
Published in final edited form as: Methods Enzymol. 2009;464:3–17. doi: 10.1016/S0076-6879(09)64001-5

Figure 1.3.

Figure 1.3

A liposome containing multiple Z rings (arrowheads) before bleach shows recovery of HccA fluorescence both outside and inside. FtsZ-YFP-mts fluorescence is recovered on the outside surface of the liposome, but no fluorescent Z rings recover inside. The exposure of fluorescence images is kept at the same level from prebleach to postbleach. (D) We did not detect any fluorescent Z rings even when we optimized the contrast in this specific panel.