Figure 1.4.

A liposome with Z rings inside shows permeability to both FtsZ-YFP-mts and to HccA. (A) Arrowheads indicate Z rings prebleach. (B) YFP fluorescence is completely eliminated on the outside and inside of the liposome at 1 min after bleach. (C) About 12 min later YFP fluorescence has partially recovered on the outside, and fluorescent Z rings have formed on the inside, showing that unbleached FtsZ-YFP-mts has entered the leaky liposome. (D) At 45 min fluorescent Z rings have spread more toward the bottom. (E, F) About 20 s after bleaching, HccA has begun to enter the liposome, apparently through a hole at the top (note the zone of brighter fluorescence in the top 1/5 of the liposome). (G, H) At 12 min HccA fluorescence has fully recovered inside compared to the prebleached image in (E). Note that the FtsZ rings recover only at the top, near the hole, at 12 min. At 45 min they have spread through the top half of the liposome.