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. 2012 Jun 20;32(25):8696–8702. doi: 10.1523/JNEUROSCI.1692-12.2012

Figure 1.

Figure 1.

Deletion of NR2B in adult-born GCs does not affect neurogenesis in the DG. A, B, Three-mouse design using an inducible reporter, EYFP, to measure GC survival and TMX-induced recombination (A) with representative images of YFP and DCX expression 6 weeks after TMX injection (B). Scale bar, 100 μm. C, Total number of adult-born GCs did not differ between NR2B-deficient and WT littermate controls 6 weeks after TMX injection, as measured by total numbers of EYFP cells. D, Genetic and experimental timeline for neurogenesis measurement. E, No effect on cell survival as measured by BrdU number at specified times postinjection. F, Representative images for BrdU (green, NeuN; red, BrdU; white, GFAP) and DCX experiments. Scale bar: top, 30 μm; bottom, 100 μm. G, No effect of treatment on total Ki67-immunoreactive cells. H, No significant difference in phenotype of BrdU cells 8 weeks after induction. I, J, No significant difference in total number of DCX cells or DCX+ cells with tertiary dendrites. K, L, Sholl analysis revealed a decrease in dendritic complexity in adult-born GCs lacking NR2B. K, Representative images and tracings. Scale bar, 20 μm. Data are mean ± SEM.