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. 2011 Nov 24;61(8):1146–1153. doi: 10.1136/gutjnl-2011-300695

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Analysis of loss of local barrier function and prediction of relapse of inflammatory bowel disease (IBD). Dextran movement (Luminal FITC-dextran (MW 4000) and intravenous Alexa-dextran 647 (MW 10 000)) through small intestinal epithelium at the site of epithelial cell shedding from luminal perfusion solutions of either 300 mOsm/l (black) and 246 mOsm/l (grey) solutions. (A) Percentage of events that show no dextran movement (sealed) and dextran leakage (leakage) in either the inward or the outward directions. (B) Percentage movement of dextran in an inward, outward or inward and outward direction from the leakage group. *p<0.05, **p<0.01, error bars show SEM (n=4 mice per group). (C) Kaplan–Meier plot of relapse of IBD patients over 12 months after confocal laser endomicroscopy stratified according to their Watson grade.