Figure 3.

The CDE motifs in the survivin core promoter do not play a major role in YM155-mediated inhibition of survivin promoter activity: Subconfluent EKVX cells were transfected with the wild type pLuc-269 construct (control) or with pLuc-269 constructs containing various mutations in CDE motifs for overnight. Cells were then treated with and with YM155 (10 nM) for 24 hours, followed by cell lysis and luciferase activity assays. The data derived from YM155-untreated cells in triplicate were divided by the data derived from YM155-treated cells in triplicate as relative fold decrease. Each bar is the fold decrease from the mean data of comparable constructs in triplicates shown in A and B. Variations from both YM155 treated and untreated assays before division were within 15%.