Figure 4.

Both consensus Sp1 and Ap2 DNA-binding oligonucleotides can compete the DNA-protein complexes formed on SCPO-4 (-149 to -109) and SCPO-5 (-113 to -67) probes: A. Relevant Ap2-like and Sp1 DNA-binding sites in SCPO-4 and SCPO-5 (bold) in the human survivin 269 bp core promoter are underlined. Of note, the AT rich area appears to be a weak Ap2 binding site. B. EMSA experiments were performed as described in the Methods section. Nuclear extracts (NE) were isolated from EKVX cells treated with and without YM155, and mixed with ³²P-labelled SCPO-4 or SCPO-5 probes in the presence and absence of consensus cold Sp1 or Ap2 binding motif oligonucleotides as shown. DNA/protein complexes were separated on a 5% non-denatured PAGE gel and visualized by autoradiography.