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. 2012 May 18;3(2):179–197.

Figure 7.

Figure 7

The Ap2 antibody failed to supershift a band in the SCPO5-protein complex (B and C), while it supershifted astrong band for the consensus Ap2 probe-protein complex (A): EMSA procedures were described in the Methods. A.Positive controls for EMSA supershift experiments. B and C. Two different EMSA supershift experimental conditionsfor the SCPO-5 probe were used. Both conditions produced a strong supershift band by Sp1 antibodies, but not Ap2antibodies. p: PC-3 cell nuclear extracts; e: EKVX cell nuclear extracts; d: DMSO; a: Ap2 antibody; a/pe: Ap2 antibodywith Ap2 inhibitory peptides; s: Sp1 antibody; Ab: antibody; nucl: nuclear; and IgG: normal IgG. D. YM155 does notdirectly bind to the SCPO-4 and SCPO-5 DNA elements. The probes, the presence and absence of YM155 in mM areindicated. YM155 interaction with SCPO-4 and SCPO-5 DNA were determined using 15% gel instead of 5% for DNA-druginteraction. D: DMSO solution (control for YM155).