Figure 8.

Identification of critical bases in the SCPO4 DNA probe: Oligonucleotides with bp mutations in SCPO-4 and 5 were used as cold competitors in EMSA experiments A. Comparison of the competition ability between wild-type (wt) SCPO-4 and its mutants (SCPO-4m1, m2, m3 and m4) in competitive EMSA using nuclear extracts isolated from PC-3 cells. B. Comparison of the competition ability between wt SCPO-5 and its mutants (SCPO-5m1, m2 and m3) in competitive EMSA using nuclear extracts isolated from PC-3 cells. scra: scramble DNA; 4: SCPO4; 4m1: SCPO4m1; 4m2: SCPO4m2; 4m3: SCPO4m3; 4m4: SCPO4m1; 5: SCPO5; 5m1: SCPO5m1; 5m2: SCPO5m2; 5m3: SCPO5m3.