Table 1.
Summary of oligonucleotides used in EMSA experiments.
| Name | DNA sequence (only forward chains are shown) | Position |
|---|---|---|
| SCPO1 | GCGTTCTTTGAAAGCAGTCGAGGGGGCGCTAGGTGTGGGCAGGGA | -267 to -222 |
| SCPO2 | GGGACGAGCTGGCGCGGCGTCGCTGGGTGCACCGCGACCACGGGCAGAG | -226 to -177 |
| SCPO3 | GGGCAGAGCCACGCGGCGGGAGGACTACAACTCCCGGCACACCC | -185 to -141 |
| SCPO4 | GCACACCCCGCGCCGCCCCGCCTCTACTCCCAGAAGGCCG | -149 to -109 |
| SCPO4m1 | GCACACCaaGCGCCGCCCCGCCTCTACTCCCAGAAGGCCG | |
| SCPO4m2 | GCACACCCCGatCCGCCCCGCCTCTACTCCCAGAAGGCCG | |
| SCPO4m3 | GCACACCCCGCGCCGCCaatCCTCTACTCCCAGAAGGCCG | |
| SCPO4m4 | GCACACCCCGatCCGCCaatCCTCTACTCCCAGAAGGCCG | |
| SCPO5 | GCCGCGGGGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATG | -113 to -67 |
| SCPO5m1 | GCCGatGGGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATG | |
| SCPO5m2 | GCCGCGttGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATG | |
| SCPO5m3 | GCCGCGGGttGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATG | |
| SCPO5m4 | GCCGCGGGGGGTGGACataaTAAGAGGGCGTGCGCTCCCGACATG | |
| SCPO5m5 | GCCGCGGGGGGTGGACCGCCTccGAtttCGTGCGCTCCCGACATG | |
| SCPO5m6 | GCCGCGGGGGGTGGACCGCCTAAGAGGGatTtaGCTCCCGACATG | |
| SCPO5m7 | GCCGCGGGGGGTGGACCGCCTAAGAGGGCGTGCGCTaaatACATG | |
| SCPO-6 | GACATGCCCCGCGGCGCGCCATTAACCGCCAGATTTGAAT | -74 to -34 |
| SCPO-7 | GAATCGCGGGACCCGTTGGCAGAGGTGGCGGCGGCGGC | -38 to +1 |
| Sp1 | ATTCGATCGGGGCGGGGCGAGC | N/A |
| Ap2 | GATCGAACTGACCGCCCGCGGCCCGT | N/A |
1) SCPO, Survivin Core Promoter Oligonucleotides. 2) Oligonucleotides for Sp1 and Ap2 binding are the consensus DNA sequences for Sp1 and Ap2. 3) SCPO4m1, Ap2 mutant oligo; SCPO4m2, left side Sp1 site mutant oligo; SCPO4m3, right site Sp1 site mutant oligo; and SCPO4m4, mutant oligo in both Sp1 sites. 4) SCPO5m1, Ap2 mutant 1; SCPO5m2, Ap2 mutant 2; and SCPO5m3, Ap2 mutant 3. Names in bold are critical mutations.