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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Int J Cancer. 2011 May 9;130(3):544–554. doi: 10.1002/ijc.26015

Figure 5. Nuclear Achn enhances MMP-9 and VEGF expression and induces luciferase activity of MMP-9 promoter.

Figure 5

A. MDA-MB-231 cells expressing ectopic Achn-GFP fusion proteins were incubated in serum-free medium for 24 hr and then the conditioned medium was collected to evaluate expression levels of MMP-9 and VEGF, where cell lysates were subjected for actin expression by immunoblotting. Arrow indicates proteolytically processed MMP-9. B. The first 710 nucleotides 5’ to the translation initiation site of human MMP-9 were introduced upstream of a luciferase reporter gene and the resulting plasmid was transfected into MDA-MB-231 cells ectopically expressing different versions of Achn. After 48 hr, cells were lysed and luciferase activity measured and normalized to the levels of lysate protein. (Mean ± SE, n=3). * = P<0.05. Vector control activities in any group were below 2000 units/mg protein (data not shown).