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. 2012 Jun 20;32(25):8521–8531. doi: 10.1523/JNEUROSCI.1245-12.2012

Figure 6.

Figure 6.

Impaired activity-dependent downregulation of mIPSCs and GAD65 in Gad1−/− neurons. a, Representative traces showing that chronic application of TTX in WT cultures reduced mIPSC amplitude and frequency. Gad1 deletion attenuated the TTX-induced downregulation of mIPSC amplitude and prevented the change in frequency. b, Summary of all data (n = 6). c, Representative images (low and high magnifications) showing the regulation of GAD65 expression by TTX in WT and Gad1−/− cultures. The intensity level of GAD65 in Gad1−/− was similar to that of WT (p > 0.08). While TTX reduced GAD65 levels in WT cultures, this downregulation was attenuated in Gad1−/− neurons. In contrast, TTX-induced downregulation of GAD65 density is completely abolished in Gad1−/− neurons compared to WT. The effect of Gad1 deletion on GAD65 puncta intensity and density mirrors that of mIPSC amplitude and frequency, respectively. d, Summary of the analysis of GAD65 intensities. WT, n = 180 synapses; Gad1−/−, n = 240 synapses. *p < 0.05; **p < 0.01; ***p < 0.001; #p < 0.001 versus control (Ctrl; no TTX). norm., Normalized.