Figure 7.
Experience regulates inhibitory transmission and GAD67 expression in the OB in vivo. a, Schematic diagram of the glomerular circuit in the OB. Olfactory sensory neurons (OSNs) release glutamate to activate ET and mitral cells. PG cells release GABA and modulate synaptic transmission in the glomerular layer (GL). b, Seven to ten days after injection of dichlobenil to ablate OSNs and reduce odor-evoked activity, olfactory marker protein (OMP) staining was largely absent in the olfactory nerve layer (ONL) and the GL as compared to DMSO controls; suppressed olfactory activity was also reflected by the reduction in tyrosine hydroxylase (TH) staining in PG cells. EPL, External plexiform layer. c, Activity deprivation by dichlobenil reduced the amplitude of sIPSCs in ET cells using whole-cell patch-clamp recordings with a Cs+ gluconate internal solution (Vh = 0 mV). d, Cumulative distributions of sIPSC amplitude in control (black) and deprived (red) conditions (n = 7). e, Sensory deprivation in the OB reduced levels of GFP in PG cells and synaptic GAD67 surrounding ET cells. White dashed line, Glomeruli; yellow rectangle, magnified region. f, Summary of effects by activity deprivation. GFP, n = 170; GAD67, n = 210. **p < 0.01; ***p < 0.001.