Figure 1.

Pds5 is required for Psm3^K106 acetylation. (A) GFP-tagged Psm3 was immunopurified from cycling cells and probed with anti-Psm3^K106Ac antibodies. (B) Cells were arrested in G1 by nitrogen starvation and released into rich medium. Progression into S-phase was monitored by measuring DNA content by flow cytometry. Total protein extracts were probed with anti-Psm3^K106Ac antibodies and the total amount of Psm3 with anti-Psm3 antibodies. (C) DNA content analysis of cycling cells at 30 °C and after 4-h incubation with hydroxyurea. Total protein extracts were probed with Psm3^K106Ac and Psm3 antibodies. The short exposure time shows a slight increase in Psm3 acetylation in the clr6-1 mutant, as reported [19]. The longer exposure (15 min) shows that Psm3 acetylation remains undetectable in a pds5Δ background. GFP, green fluorescent protein; Hu, hydroxyurea; IP, immunoprecipitation; Wt, wild type.