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. Author manuscript; available in PMC: 2012 Jul 3.
Published in final edited form as: Cell Rep. 2012 May 10;1(5):424–433. doi: 10.1016/j.celrep.2012.04.001

Figure 1. The Absence of the mcm5s2 Modification Leads to Specific Phenotypes Suppressed by Overexpression of the Unmodified tRNALysUUU.

Figure 1

(A) Spot dilution assay of wt, Δelp3 and Δctu1Δelp3 strains grown on rich media at 32°C or 36°C, or supplemented with 1 M sorbitol, 5 ng/ml rapamycin, or 0.005% SDS.

(B) Left panel: DAPI and calcofluor stained Δelp3 or Δctu1Δelp3 cells grown at 36°C. Right panel: measurement of septated cell size (reported to wild-type = 100%; p value by Student’s t test; n > 100) and counting of misplaced septa (n > 100) in the Δelp3 and Δctu1Δelp3 strains.

(C) Spot dilution assay of wt, Δctu1Δelp3, cdc2-33 and Δctu1 Δelp3 cdc2-33 grown on rich media at 25°C, 32°C, or 36°C and a summary of similar interactions with the cdc25-22 and cdc13-117 alleles.

(D) Spot dilution assay of the Δctu1Δelp3 strain expressing elevated levels of tRNALysUUU, tRNAGlnUUG, tRNAGluUUC, or tRNALysCUU on indicated selective minimal media.

See also Figures S1 and S2.