(A) Spot dilution assay of wt, Δelp3 and Δctu1Δelp3 strains grown on rich media at 32°C or 36°C, or supplemented with 1 M sorbitol, 5 ng/ml rapamycin, or 0.005% SDS.
(B) Left panel: DAPI and calcofluor stained Δelp3 or Δctu1Δelp3 cells grown at 36°C. Right panel: measurement of septated cell size (reported to wild-type = 100%; p value by Student’s t test; n > 100) and counting of misplaced septa (n > 100) in the Δelp3 and Δctu1Δelp3 strains.
(C) Spot dilution assay of wt, Δctu1Δelp3, cdc2-33 and Δctu1 Δelp3 cdc2-33 grown on rich media at 25°C, 32°C, or 36°C and a summary of similar interactions with the cdc25-22 and cdc13-117 alleles.
(D) Spot dilution assay of the Δctu1Δelp3 strain expressing elevated levels of tRNALysUUU, tRNAGlnUUG, tRNAGluUUC, or tRNALysCUU on indicated selective minimal media.
See also Figures S1 and S2.