Figure 2.

Quaternary structure. (a) Elution profile of T. aquaticus and pig GTP-specific SCS (5 mg ml⁻¹ each) from gel-filtration chromatography in 5 mM 2-mercaptoethanol, 0.1 mM EDTA, 60 mM potassium phosphate pH 7.4. (b) Sedimentation equilibrium of T. aquaticus SCS under native conditions using 1.77, 1.31 and 0.8 mg ml⁻¹ in 2 mM dithiothreitol, 20 mM potassium phosphate pH 7.4.