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PCR and RT-PCR analyses of LOS5 gene in transgenic cotton. (A) PCR analysis using specific primers for the LOS5 gene to identify two independent transgenic lines. Lane M, DNA marker; lane P, positive control (plasmid pCAMBIA1300-LOS5); Z35, non-transgenic control; lanes L5 and L8, transgenic lines. (B) RT-PCR analysis of LOS5 gene expression in the two transgenic lines under normal and water-deficit conditions. The UBQ7 gene was used as an internal control.
