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. 2012 Mar 22;63(10):3923–3934. doi: 10.1093/jxb/ers083

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Immunogold localization of γ-glutamylcysteine synthetase (γECS) in roots, nodules, and leaves of several legumes. (A) Immunogold-labelled amyloplast (arrow) in a bean root tip, including labelling of starch grains (arrowheads). (B) Immunogold-labelled plastids (arrows) in a bean nodule. (C) Immunogold-labelled plastids (arrows) in a Sesbania rostrata root nodule. (D) Immunogold-labelled chloroplast (arrow) in a S. rostrata stem nodule. (E) Immunogold-labelled chloroplast (arrows) in an alfalfa leaf; note the relatively high-density labelling of the starch grains (arrowheads). (F) Plastids in a bean root tip treated with preimmune serum substituted for the γECS antibody (negative control). cyt, cytoplasm; ch, chloroplast; g, golgi; is, intercellular space; m, mitochondrion; n, nucleus; p, plastid; px, peroxisome; s, starch grain; v, vacuole. Bars, 1 μm.