Fig. 4.

Overexpression and knockdown of OsERF922 alter resistance to Magnaporthe oryzae and the expression of defence-related genes. (A–H) Analysis of OsERF922 expression in OsERF922 overexpression (S6, S13, S20, S28, S59, and S63) and knockdown (D1, D2, D30, D46) rice lines by Western blot (A) and quantitative reverse-transcription PCR (B, OsERF922; C, PR1a; D, PR1b; E, PR10; F, PAL; G, OsCPS2; H, OsCPS4), as described in Fig. 1. Total proteins were extracted from leaves of 7-d-old seedlings of the transgenic and wild-type plants, separated on a 10% SDS-PAGE, and detected by the mouse anti-Flag antibody. Three-week-old transgenic and the wild-type plants were inoculated with the virulent M. oryzae P140 isolate and several leaves from each line were sampled for analysis of gene expression 6 h post inoculation. Data are representative of two independent biological replicates. (I) Transgenic plants of OsERF922 were tested for resistance to M. oryzae. Leaves of 3-week-old rice plants were inoculated with conidial suspensions of M. oryzae (10⁵ conidia ml⁻¹) isolate P140. Leaves were detached from inoculated plants 7 d post the infection and photographed. Experiments were repeated three times with similar results. Bar, 1 cm. (J) Histograms showing the area of lesions formed on the third leaves (15 cm in length) of 20 plants for each line. Values marked with different letters are significantly different (P < 0.01, Fieldman Rank Sums test).