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. 2012 Jun 27;32(26):8865–8870. doi: 10.1523/JNEUROSCI.0937-12.2012

Figure 2.

Figure 2.

NS5A1b inhibits Src, but not p38, phosphorylation of Kv2.1. A, Top, Whole-cell currents (−80 to +30 mV step) in CHO cells expressing Kv2.1, Kv2.1(S800E), or Kv2.1 (Y124F) together with either VEC or NS5A1b (marked by a +). Scales are 5 nA and 5 ms. Bottom, Average K+ current suppression by NS5A1b coexpression, expressed as a percentage of the average currents present in vector-expressing cells; n = 9–19 cells per group (42 cells total); *p < 0.05, significantly different from the other two groups, ANOVA/Dunnet. B, CHO cells were cotransfected with Kv2.1(S800A), Kv2.1, p38, NS5A1b or vector. Kv2.1 immunoprecipitates were run by SDS-PAGE and probed with a phospho-specific antibody recognizing phosphorylated residue S800. Top, Representative immunoblot; Bottom, summary and quantification of results in three independent experiments, normalized to total Kv2.1 protein; **p < 0.01, ANOVA/Dunnet versus Kv2.1 control (third bar). C, CHO cells were cotransfected with Kv2.1, Src, NS5A1b or vector. Kv2.1 immunoprecipitates were run by SDS-PAGE and probed with a phosphotyrosine antibody. Top, Representative immunoblot; Bottom, summary and quantification of results in three independent experiments; **p < 0.01, ANOVA/Bonferroni versus Src/vector group (second vs third bar).