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. 2010 Nov 17;25(1):157–169. doi: 10.1210/me.2010-0308

Figure 4.

Figure 4

Sequence-, fragmentation-, and differentiation-induced activation of CRFR2β 5′-flanking region. A, Genomic sequence of mCRFR2β 5′-flanking region. 5′-Untranslated region is shown in italic letters. The six primers used for promoter fragmentation are underlined, and putative sites for muscle-specific transcription factors, recognized using TESS (Transcription Element Search System), are indicated. B, Electrophoretic analysis of pGL3-basic vectors containing truncated mCRFR2β 5′-flanking region. C, Activity of CRFR2β fragmented promoter during myogenic differentiation. C2C12 cells were transfected with the reporter plasmid preceded by the different 5′-flanking region fragments. Luciferase activity during the myogenic differentiation in 2% horse serum containing DM was determined. The relative luciferase activity was corrected to β-gal activity (results are shown as fold increase over the basal activity of each fragment, shown as mean ± sem). AS, Antisense.*, P < 0.05; **, P < 0.001 vs. the full fragment.