Table 2. Inhibition of LmCS by peptides corresponding to the C-termini of the L. major and A. thaliana SATs.
Results in bold represent the mean ± standard deviation of three independent determinations. All other results are from a single IC50 curve. ND, not determined.
| AtOAS-TL IC50 (µM) | LmCS IC50 (µM) | |
|---|---|---|
| Peptides | ||
| DYVI | 8 ± 3 | 7 ± 1 |
| GSGI | ≤2000 | 1500 |
| DGSGI | 170 | 620 ± 50 |
| EGDGSGI | 240 | 270 ± 70 |
| N-blocked amino acids | ||
| L-Isoleucine | ND | >4000 |
| N-Benzyl-L-isoleucine | ND | >1000 |
| N-3-Indolylacetyl-L-isoleucine | ND | 260 |
| N-CBZ-L-isoleucine | ND | 320 |
| N-CBZ-L-valine-isoleucine | ND | 1410 |