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Purification of TgISP1-2 by gel-filtration chromatography on a Superdex 75 16/60 HiLoad column. Standards (plotted against secondary axis, dotted curve): peak I, carbonic anhydrase (29 kDa); peak II, ribonuclease A (13.7 kDa); peak III, aprotinin (6.5 kDa). TgISP1-2 (solid purple curve) elutes as a monomer with conformational impurity. Inset: SDS–PAGE analysis of a representative fraction showing a low apparent molecular weight of 7 kDa (the expected molecular weight is 13.9 kDa).
