Figure 6.
Ribozyme targeted regions. Stable high frequency recurrent stem loop structural motifs containing single stranded annealing platforms were selected as suitable candidate platforms for hhRz targeting. The 33 nt ss loop in region 250 extends from nts 249–281 in the human RHO mRNA sequence (NM000539). The 8 nt ss loop in region 780 extends from nts 780–787. The 39 nt ss loop in region 1190 extends from nts 1182–1220 and the 12 nt ss substate loop extends from nts 1178–1189. The 14 nt ss loop extends from nts 1406–1419. Available hhRz cleavage sites within these structural motifs are shown by arrows. The NUH↓ sites targeted in this study are shown by arrowheads.