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. 2010 Mar 28;10:26. doi: 10.1673/031.010.2601

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© 2010

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Agarose gel showing PCR results using the ITS2 primer set (sdi-16F/sdi-20R) designed for the detection of Scirtothrips dorsalis. Duplicate reactions were performed from single individuals representing seven thrips species. The marker fragment (131–135 bp) was successfully amplified from S. dorsalis DNA (lanes 14 and 15), while there was no amplification from the other thrips species (lanes 2 to 13). Lanes 1 and 17, 100 bp DNA ladder; Lanes 2 and 3, T. palmi; Lanes 4 and 5, T. tabaci; Lanes 6 and 7, S. citri; Lanes 8 and 9, F. occidentalis; Lanes 10 and 11, S. perseae; Lanes 12 and 13, F. schultzei; and Lane 16, negative control. High quality figures are available online.