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. Author manuscript; available in PMC: 2012 Jul 3.
Published in final edited form as: Exp Eye Res. 2011 Jan 21;92(5):328–337. doi: 10.1016/j.exer.2011.01.004

Fig. 2. High throughput cytotoxicity assay.

Fig. 2

HEK293S-MSR1 cells were transfected with the PTGS agents Rhoi2, Rhoi725, Prislei-725HH16, and pUC-CELO-725HH16, as well as the control vectors pUC-VAI, Prislei-VAI, Rhoiscramble, and pUC-CELO, and later stained with SYTOX Green at 72 hrs post-transfection. SYTOX Green Fluorescence from a representative non-transfected cell sample (A), a cell sample transfected with Prislei-725HH16 (B), and after cell surface permeabilization of the non-transfected (C) and transfected (D) samples with saponin.