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. Author manuscript; available in PMC: 2012 Jul 3.
Published in final edited form as: Exp Eye Res. 2011 Jan 21;92(5):328–337. doi: 10.1016/j.exer.2011.01.004

Fig. 3. PTGS agents do not exert cytotoxicity in HEK293S-MSR1 cells.

Fig. 3

Cell death in response to transfection with VAI-hhRz chimeras and shRNAs was assayed by staining dead cells with SYTOX Green nucleic acid stain and measuring SYTOX Green fluorescence 72 hours post-transfection. Cells undergoing apoptosis have permeable cell surface membranes and take up SYTOX Green which binds to nuclear DNA to result in strong enhanced fluorescence emission. After 1 hr incubation with 1% saponin to permeabilize all cell membranes, an estimate of total cell number was determined by again measuring SYTOX Green fluorescence emission. The VAI-hhRz chimeras and shRNA constructs did not cause any significant cell death when compared to the non-transfected control (ANOVA p= 0.69484).