Figure 2.

Expression of heparanase in human retinal vascular endothelial cells is suppressed by RNAi. A: Human retinal vascular endothelial cells (HRECs) were infected with Con-LV or heparanase small interfering RNA recombinant lentiviral vector (HPSE-LV) at a multiplicity of infection (MOI) of 60. Green fluorescent protein (GFP) expression and phase contrast images were captured after 72 h (original magnification × 200). B: The heparanase mRNA levels were detected using real-time PCR after the HRECs were treated with HPSE-LV and Con-LV. HPSE-LV significantly inhibited the expression of HREC heparanase mRNA (●p<0.01, compared with HRECs; ▲p<0.01, compared with the Con-LV group). Each group experiment was repeated 3 times (n=3). C: western blotting analysis showed that the heparanase protein was markedly inhibited by HPSE-LV in HRECs.