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. 2012 Jun 20;18:1658–1667.

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Copyright © 2012 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of matrix metalloproteinase 9 (MMP-9) antibody (Cell Signaling Cat# 3852, Lot#2) via western blot. The molecular weight of MMP-9 is 84, 92 kDa. Lanes 1–3 go Left to Right. Lane 1: is a Magic Mark Western Standard (Cat # LC5602, Life Technologies) molecular weights ranging from 20 to 220 kDa. Lane 2, shows the validation of the MMP-9 antibody, using human endothelial cell lysates (BD Cat# 611450, Lot: 86722). Lane 3 shows is a negative control loaded with BSA (BSA; Thermo Fisher Product # 23209).