Table 3. Variability within Individual Laboratory Processes.

Method and Variable	n	p value1	% Variance
RNA Extraction
RNA Extraction Machine	4	0.843	0.10%
RNA Extraction Kit Lot	26	<2×10−16	18%
RNA Operator	6	0.517	0.40%
cDNA Synthesis
cDNA Synthesis Machine	4	0.074	1.6%
cDNA Kit Lot	6	8.5×10−11	6.9%
cDNA Operator	5	0.012	0.8%
Sample Addition to Assay Plate
Sample Addition Machine	5	0.149	0.8%
Sample Addition Operator	8	0.358	0.9%
qRT-PCR
qRT-PCR Machine	6	0.032	1.3%
qRT-PCR Assay Plate Lot	24	<2×10−16	21.8%
qRT-PCR Operator	6	0.016	1.5%

¹p values in bold are <0.05.