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. 2012 Jul 3;7(7):e40301. doi: 10.1371/journal.pone.0040301

Figure 1. Effect of Ac-SDKP on expression of TGF-β receptor type I and II, collagen type I and III in cultured rat fibroblasts.

Figure 1

TGF-β treatment of cultured rat fibroblasts enhanced protein and mRNA levels of TGF-β receptor and increased collagen synthesis, while pretreatment with Ac-SDKP and LY364947 markedly attenuated the effects of TGF-β1. A) Western blot analysis with antibodies against TGF-β receptor type I and II, collagen type I and III; C: control group; T: TGF-β group; T+ LY: TGF-β+ LY364947 group; T+ Ac: TGF-β+ Ac-SDKP group. B) The TGF-β receptor type I protein expression measured by Western blot; C) The TGF-β receptor type II protein expression measured by Western blot; (D) The collagen type I protein expression measured by Western blot; (E) The collagen type III protein expression measured by Western blot; F) The collagen type I mRNA expression measured by Real-time PCR; G) The collagen type III mRNA expression measured by Real-time PCR.