Figure 4. Sodium tungstate has an effect on the CRMP2 protein.

An important portion of all spots isolated were identified as CRMP2. (A) Representative ARC 2D gel from treated lean mouse. Insert image shows different CRMP2 proteins. Numbers correspond to identification of CRMP2 by mass spectrometry. (B) Protein identification using mass spectrometry was verified performing 2D-Western blot (insert image). Spots identified as CRMP2 by mass spectrometry co-localized perfectly with those identified by 2D-western blot, confirming that identification had been performed correctly. (C) p-CRMP2^T514 antibody reveals that a significant number of posttranslational changes of this protein involve phosphorylation, and the great pI variability indicates that multiple posttranslational changes occur all at once. (D) Representative close-up images of spots with different pI corresponding to CRMP2.