Table 1. Tungstate targets identified by proteomics in different hypothalamic nuclei.

Spota	Uniprot	Protein	Phenotypeb	Ratioc	P	Nuclei
Axonal growth and neuronal plasticity
213	O08553	CRMP2	L	1.61	0.011	ARC
215	O08553	CRMP2	L	1.34	0.012	ARC
244	O08553	CRMP2	L	−1.19	0.031	ARC
297	P46660	Alpha-internexin	L	−1.8	0.024	ARC
62	P08553	NEFM	O	1.5	0.037	ARC
1475	P03995	GFAP	O	1.21	0.017	ARC
242	O08553	CRMP2	L	1.34	0.021	PVN
278	P46660	Alpha-internexin	O	−1.1	0.022	PVN
217	O08553	CRMP2	L	−1.32	0.061	LHA
219	O08553	CRMP2	L	−1.41	0.029	LHA
224	O08553	CRMP2	L	−1.61	0.006	LHA
176	O08553	CRMP2	O	1.19	0.059	LHA
Cytoskeleton and neuronal structure
1475	P60710	Beta-actin	O	1.21	0.017	ARC
1559	P60710	Beta-actin	O	1.63	0.028	ARC
1058	Q8BXF8	Actin-relatedprotein M1	O	−1.21	0.047	ARC
1224	P68369	Alpha-tubulin,chain 1A	O	1.61	0.013	ARC
636	A2AQ07	Tubulin beta1	L	−1.81	0.038	LHA
Energy metabolism
415	P14152	Malate dehydrogenase	L	−1.21	0.044	ARC
479	P14152	Malate dehydrogenase	L	1.2	0.027	ARC
1419	P17182	Enolase 1	L	1.36	0.04	ARC
1224	Q8BKZ9	Pyruvate dehydrogenase	O	1.61	0.013	ARC
277	Q9DCT2	NADH dehydrogenase	L	1.72	0.015	PVN
329	Q9DCT2	NADH dehydrogenase	L	1.32	0.02	PVN
303	P17751	Triosephosphate isomerase	O	−1.82	0.023	PVN
567	Q811J3	Aconitase 2	O	−1.77	0.034	PVN
359	Q00623	Apolipoprotein A-I	O	−1.6	0.002	PVN
431	Q04447	Creatine kinase B	L	1.14	0.003	ARC
361	P21550	Enolase 3	L	−1.21	0.028	LHA
373	P17182	Enolase 1	L	−1.62	0.05	LHA
518	P14152	Malate dehydrogenase	L	−1.21	0.023	LHA
664	Q9D051	Pyruvate dehydrogenase-β	L	−1,42	0,049	LHA
230	P52480	Pyruvate kinase	O	1.43	0.015	LHA
1475	Q04447	Creatine kinase B	O	1.21	0.017	ARC
648	P35486	Pyruvate dehydrogenase-α	O	1.61	0.059	LHA
Others
98	P48722	Hspa 4L	L	1.19	0.021	ARC
1604	Q9Z2Q6	Septin-5	L	1.33	0.036	ARC
1224	P62814	Vacuolar H+ATPase B2	O	1.61	0.013	ARC
1058	P31938	MEK1	O	−1.21	0.047	ARC
141	P50518	V- ATPase E1	L	−1.17	0.046	PVN
242	P50516	V-ATPase A	L	1.34	0.021	PVN
329	P70202	Latexin	L	1.32	0.02	PVN
428	Q9R0Y5	Adenilate kinase 1	O	−1.77	0.009	PVN
559	P09671	Superoxide dismutase	O	−1.21	0.047	PVN
149	P63017	Hspa8	L	−1.51	0.052	LHA
664	P62880	Gnb 2	L	−1.42	0.049	LHA

^aSpot numbers corresponding to 2D images for each type of nuclei (Figure 2).

^bMice phenotypes: L  =  lean. O  =  obese.

^cRatios of protein expression levels calculated using DeCyder software as the fold change in normalized spot volume comparing tungstate treated and non treated animals (Student’s t test based on the log of the ratio of the treated group to the control group).