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. 2012 Jul 3;10(7):e1001357. doi: 10.1371/journal.pbio.1001357

Figure 4. Bam blocks nuclear accumulation of pMad and dSmurf degrades cytoplasmic pMad.

Figure 4

(A,B) BMP signaling in wild-type germaria visualized with anti-pMad antibody (A) or by Dad-lacZ expression (B). (C) A tumorous TkvAct germarium with BMP signaling in all germ cells, as evidenced by Dad-lacZ expression. (D–H) Flies of genotype TkvAct (D), hs-bam TkvAct (E), hs-bam (F), dSmurf15C (G), or hs-bam dSmurf15C (H) were subject to a 2-h heat shock at 37° and a 4-h recovery at room temperature. (D) A TkvAct ovariole with pMad staining in germ cell nuclei, indicated by absence of Vasa staining. (E) A hs-bam; TkvAct ovariole with decreased nuclear localization of pMad and increased cytoplasmic pMad. (F) A hs-bam germarium with no pMad staining visible in GSCs. (G) A dSmurf15C germarium with both nuclear and cytoplasmic pMad staining in GSCs, and cytoplasmic pMad staining in Cbs and germline cysts. (H) A hs-bam; dSmurf15C germarium with loss of pMad nuclear staining but retention of cytoplasmic pMad staining in GSCs. (A–H) Arrow, CpC niche; solid line, CpC-GSC interface. (A–B,F–H) Dashed outline, individual GSC. (B) Solid circle, a Cb with low levels of β-galactosidase staining. (F–H) Inset: white, anti-pMad staining; asterisk, GSC nucleus.