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. 2012 Jul 3;7(7):e40258. doi: 10.1371/journal.pone.0040258

Figure 2. 8,9-EET attenuated B-cell proliferation and survival.

Figure 2

A. Splenic B cells were prelabeled with carboxyfluorescein succinimidyl ester (CFSE) and stimulated with 5 µg/ml LPS with or without 8,9-EET (1 µM) for 72 hr. Cell proliferation was monitored by measuring the dilution of CFSE. B. Quantification of 3 independent experiments of proliferation assay. C. Splenic B cells were stimulated with 5 µg/ml LPS with or without 8,9-EET (1 µM) for 48 hr. Flow cytometry of cell survival after annexin V (AnnV) and propidium iodide (PI) staining of cultured B cells. D. Quantification of three independent experiments of survival. Data are expressed as means ± SEM. *, P<0.05 vs. no 8,9-EET.