Figure 4. 8,9-EET inhibited class switch recombination of B cells.

A. Splenic B cells were stimulated with 5 µg/ml LPS and 50 ng/ml IL-4 with or without 8,9-EET (1 µM) for 3 days. Flow cytometry of surface IgG₁ (sIgG₁) expression in cells. B. Quantification of 3 independent experiments of sIgG₁ expression in B cells. C. Real-time RT-PCR analysis of mRNA levels of AICDA, Iγ₁-Cγ₁, Iμ-Cγ₁, Iγ_2b-Cγ_2b and Iμ-Cγ_2b in 3-day culture. GAPDH was used as the control. Data are means ± SEM of three independent experiments. *, P<0.05 vs. no 8,9-EET.