Table 4. Detailed PCR-findings for recipients of organs from donors with suspected/confirmed chronic Chagas disease.
Patient ID | Patient description | time of specimen | Specimen type | results individual assays | Reported result | ||
kDNA TaqMan | TCZ TaqMan | 18 S rRNA TaqMan | |||||
10 | kidney recipient | 2 weeks post-transplant | EDTA blood | negative | negative | negative | negative |
3 weeks post-transplant | EDTA blood | positive | positive | negative | equivocal | ||
4 weeks post-transplant | EDTA blood | positive | positive | negative | equivocal | ||
5 weeks post-transplant | EDTA blood | positive | negative | negative | equivocal | ||
6 weeks post-transplant | EDTA blood | negative | negative | negative | negative | ||
7 weeks post-transplant | EDTA blood | negative | negative | negative | negative | ||
9 weeks post-transplant | EDTA blood | negative | negative | negative | negative | ||
11 weeks post-transplant | EDTA blood | negative | negative | negative | negative | ||
11 | kidney recipient | 1 week post-transplant | EDTA blood | negative | negative | negative | negative |
3 weeks post-transplant | EDTA blood | positive | positive | negative | equivocal | ||
4 weeks post-transplant | EDTA blood | negative | negative | negative | negative | ||
5 weeks post-transplant | EDTA blood | negative | negative | negative | negative | ||
3 months post-transplant | EDTA blood | negative | negative | negative | negative | ||
6 months post-transplant | EDTA blood | negative | negative | negative | negative | ||
12 | Heart recipient | 2 weeks post-transplant | EDTA blood | negative | negative | negative | negative |
3 weeks post-transplant | EDTA blood | negative | negative | negative | negative | ||
4 weeks post-transplant | EDTA blood | BC positive | BC positive | negative | positive | ||
5 weeks post-transplant | EDTA blood | positive | positive | negative | positive | ||
6 weeks post-transplant | EDTA blood | negative | negative | negative | negative† | ||
7 weeks post-transplant | EDTA blood | negative | negative | negative | negative† | ||
3 months post-transplant | EDTA blood | negative | negative | negative | negative | ||
5 months post-transplant | EDTA blood | negative | negative | negative | negative | ||
8 months post-transplant | EDTA blood | negative | negative | negative | negative |
All of the patients in Table 4 were serologically negative at time of transplant and none sero-converted during the time they were monitored at CDC.
BC = buffy coat; only the buffy coat fraction was PCR positive for these samples.
†: indicate specimens that were collected during drug treatment (benznidazole or nifurtimox). Drug treatment information is missing for patients #4 and #9.